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The tumour is a complex bio-organism. We introduce a mammalian three-dimensional tumour model in vitro and in vivo, which is based on collagen-rich tumour cell colonies and the presence of a specific tumour ECM. The fast growing tumour cell population creates an invasive front and stabilizes the tumour cell monolayer. The tumour cells produce an ECM with characteristic basement membrane (BM)-like structures and form functional vasculature. We develop a new transwell-chamber assay to study three-dimensional cell migration in vitro. We make use of the morphology of the collagen II-rich matrix. The matrix morphology requires the use of 70-100% confluent cells, which makes the assay for chemotaxis of single cells highly reproducible. The assay is also suitable for the study of non-motile cells. Permeability of the matrix is measured by means of the tracer diffusion. By using this assay, we are able to distinguish between cells with a fast single-cell migration and cells with collective migration. In an in vivo assay, we implant into nude mice a 3D tumour cell matrix consisting of colon-adenocarcinoma cell lines HT29, L23, and SW403. We apply the same assay to monitor the presence of single-cell and collective single-cell migration and invasion of these different colon carcinoma cell lines. We successfully discriminate between these two types of migration in vitro and in vivo. Our three-dimensional tumour model is a useful tool for the studies of tumour cell migration, invasion, and the role of the tumour-associated stroma.Chronic lymphocytic leukemia (CLL) has been characterized in recent years as a disease of the slowly dividing B-cells with excessive production of Ig and a reduced ability to destroy and suppress other cells. CLL is the most common hematologic malignancy in Western countries and is characterized by the accumulation of mainly monoclonal B-lymphocytes in the bone marrow, blood and lymphoid organs.
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Autocad Vectors is a runtime library and assembly (DLL) from Autodesk Vectors that allows other Autodesk software to use vector graphics and perform certain operations on them. Its purpose is to provide vectors for use in Autocad software.

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